Correlation between expression levels of serum miR-181a and S1PR1 mRNA and the occurrence of acute exacerbation in patients with bronchial asthma
10.3969/j.issn.1673-4130.2024.14.005
- VernacularTitle:支气管哮喘患者血清miR-181a、S1PR1 mRNA表达水平与急性发作期发生的相关性
- Author:
Dongxu LI
1
;
Li LIU
;
Xiao JING
;
Chunyan LIU
Author Information
1. 石家庄市急救中心中心站,河北石家庄 050000
- Keywords:
bronchial asthma;
microRNA-181a;
sphingosine 1-phosphate receptor 1;
occurrence of a-cute exacerbation
- From:
International Journal of Laboratory Medicine
2024;45(14):1687-1691,1698
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the expression levels of serum microRNA-181a(miR-181a)and sphingosine 1-phosphate receptor 1(S1PR1)messenger RNA(mRNA)and the occur-rence of acute exacerbation in patients with bronchial asthma.Methods From January 2021 to May 2023,a to-tal of 132 patients with bronchial asthma in acute exacerbation stage from Shijiazhuang Emergency Center were selected as the acute stage group,89 patients with bronchial asthma in remission stage were selected as the remission stage group and 90 healthy personnel for physical examination were selected as the control group.The acute stage group was divided into mild group(50 cases),moderate group(45 cases)and severe group(37 cases).Clinical data were collected and pulmonary function[ratio of forced expiratory volume to forced vital capacity in the first second(FEV1/FVC)and percentage of peak expiratory flow rate to expected value(PEF％pred)]was examined.Real-time fluorescence quantitative PCR was used to detect the serum miR-181a and S1PR1 mRNA expression.Pearson method was used to analyze the correlation between serum miR-181a and S1PR1 mRNA and lung function indicators in patients with acute exacerbation of bronchial asthma.Multivariate Logistic regression analysis was used to identify factors affecting the occurrence of acute exacerbation in patients with bronchial asthma.Receiver operating characteristic(ROC)curve was used to e-valuate the value of serum miR-181a and S1PR1 mRNA in predicting the occurrence of acute exacerbation in patients with bronchial asthma.Results The FEV1/FVC,PEF％pred and relative expression level of serum miR-181a in control group,remission stage group and acute stage group were decreased successively,while the relative expression level of S1PR1 mRNA were increased successively(P＜0.05).The relative expression lev-el of serum miR-181a in mild group,moderate group and severe group was decreased successively,and the rel-ative expression level of S1PR1 mRNA was increased successively(P＜0.05).In patients with acute exacer-bation of bronchial asthma,serum miR-181a and S1PR1 mRNA were negatively correlated(P＜0.05),serum miR-181a was positively correlated with FEV1/FVC,PEF％pred(P＜0.05),and serum S1PR1 mRNA was negatively correlated with FEV1/FVC,PEF％pred(P＜0.05).Low level of FEV1/FVC,low level of PEF％pred,low relative expression level of miR-181a and high relative expression level of S1PR1 mRNA were inde-pendent risk factors affecting the occurrence of acute exacerbation in patients with bronchial asthma(P＜0.05).The area under the curve(AUC)of the combination of serum miR-181a and S1PR1 mRNA for predic-ting the acute exacerbation in patients with bronchial asthma was 0.890,which was higher than the AUC of the two predicted alone(P＜0.05).Conclusion The serum miR-181a in patients with bronchial asthma is lowly expressed,while S1PR1 mRNA is highly expressed.Both are related to the occurrence and severity of acute exacerba-tion as well as lung function,and have high predictive efficacy for the occurrence of acute exacerbation.
