Effect of pristimerin on LPS-induced alveolar epithelial cell injury by regulating the CCL2-CCR2 signaling axis
10.3969/j.issn.1673-4130.2024.13.015
- VernacularTitle:扁蒴藤素调节CCL2-CCR2信号轴对LPS诱导的肺泡上皮细胞损伤的影响
- Author:
Jie CHEN
1
;
Huilin JIANG
;
Liyan WANG
;
Junjie YANG
Author Information
1. 华中科技大学协和江北医院儿科,湖北武汉 430100
- Keywords:
pristimerin;
CC chemokine ligand 2-CC chemokine receptor 2 signaling axis;
lipopolysac-charides;
pulmonary alveolar epithelial cells;
injury
- From:
International Journal of Laboratory Medicine
2024;45(13):1611-1616
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of pristimerin(PT)on lipopolysaccharide(LPS)induced alveolar epithelial cell injury by regulating the CC chemokine ligand 2(CCL2)-CC chemokine receptor 2(CCR2)signaling axis.Methods A549 alveolar epithelial cells were routinely cultured and randomly separa-ted into control group(normally cultured),LPS group(treated with 10 μg/mL LPS),PT-L group(1 μmol/L PT),PT-H group(2 μmol/L PT)and PT-H+RS504393 group(2 μmol/L PT+50 ng/mL RS504393).CCK-8 method was applied to detect cell activity.EdU experiment was applied to measure cell proliferation.Flow cytometry was applied to detect cell apoptosis.Enzyme linked immunosorbent assay(ELISA)was applied to detect the levels of inflammatory factors such as tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and IL-6 in cells.Western Blot was applied to detect the expression levels of CCL2,CCR2 and apoptosis related proteins of cells in each group.Results Compared with the control group,the absorbance(A)450(48,72 h),cell proliferation rate,and Bcl-2 protein expression level of A549 cells in the LPS group were greatly reduced(P<0.05),and the apoptosis rate,TNF-α,IL-1β,IL-6 levels,CCL2,CCR2,and Bax protein expression levels were greatly increased(P<0.05).Compared with the LPS group,the A450(48,72 h),cell proliferation rate,and Bcl-2 protein expression level of A549 cells in the PT-L and PT-H groups were greatly increased(P<0.05),and the apoptosis rate,TNF-α,IL-1β,IL-6 levels,CCL2,CCR2,and Bax protein expression levels were greatly decreased(P<0.05).The CCL2 inhibitor RS504393 promoted the improvement effect of PT on LPS induced alveolar epithelial cell injury.Conclusion PT may improve LPS-induced alveolar epithelial cell injury by down-regulating the CCL2-CCR2 signaling axis.
