Expression level of serum microRNA-373 in gastric cancer and its diagnostic value
10.3969/j.issn.1673-4130.2024.10.005
- VernacularTitle:血清微小RNA-373在胃癌中的表达水平及其诊断价值
- Author:
Shuyin DENG
1
;
Rong PU
Author Information
1. 广东医科大学第一临床医学院,广东湛江 524023
- Keywords:
microRNA-373;
gastric cancer;
expression level;
early diagnosis;
biomarker
- From:
International Journal of Laboratory Medicine
2024;45(10):1175-1179
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression level and diagnostic value of microRNA-373(miR-373)in gastric cancer.Methods A total of 80 patients with gastric cancer admitted to the Gastric Cancer Diagnosis and Treatment Center of Dongguan Third Clinical College of Guangdong Medical University from February 2021 to February 2023 were selected as the gastric cancer group.80 patients with benign gastric diseases who underwent gastroscopy in Dongguan Third Clinical College of Guangdong Medical University during the same period and did not have tumors by pathological examination were selected as the benign gastric disease group,and 80 healthy subjects who underwent the physical examination during the same period were selected as the control group.Serum samples were collected from the subjects,and the expression level of miR-373 was detec-ted by real-time quantitative polymerase chain reaction(qRT-PCR),and its correlation with clinicopathologi-cal features was analyzed.Serum carbohydrate antigen 199(CA199)and carcinoembryonic antigen(CEA)were detected by automatic chemiluminescence immunoassay.The diagnostic efficacy of miR-373,CA199 and CEA for gastric cancer was evaluated by receiver operating characteristic(ROC)curve.Results The expres-sion levels of serum miR-373,CA199 and CEA in gastric cancer group were higher than those in benign gastric disease group and control group,and the differences were statistically significant(P＜0.05).The expression level of miR-373 was closely related to tumor maximum diameter,lymph node metastasis and TNM stage in patients with gastric cancer(P＜0.05).ROC curve analysis showed that the area under the curve of miR-373,CA199 and CEA in the diagnosis of gastric cancer was(95i％CI:0.819-0.926),0.874(95％CI:0.813-0.921)and 0.836(95％CI:0.769-0.889),respectively.The diagnostic efficacy of miR-373 was better than that of traditional tumor markers CA199 and CEA.Conclusion miR-373 is highly expressed in gastric cancer tissues,and the expression level of miR-373 is related to the clinicopathological features of gastric cancer,which has high application value in the early diagnosis of gastric cancer.
