Transient receptor potential vanilloid 1-based gene therapy alleviates orthodontic pain in rats
- Author:
Guo RUI
1
;
Zhou YANG
;
Long HU
;
Shan DI
;
Wen JING
;
Hu HUIMIN
;
Yang HONG
;
Wu ZHOUQIANG
;
Lai WENLI
Author Information
1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Orthodontics
- From:
International Journal of Oral Science
2019;11(1):70-78
- CountryChina
- Language:Chinese
-
Abstract:
Orthodontic pain that is induced by tooth movement is an important sequela of orthodontic treatment and has a significant effect on patient quality of life.Studies have shown that the high expression of transient receptor potential vanilloid 1 (TRPV1) in trigeminal ganglions plays a vital role in the transmission and modulation of orofacial pain.However,little is known about the role of TRPV1 in orthodontic pain.in this study,male Sprague-Dawley rats were randomly assigned to six groups to study the role of TRPV1 in the modulation of tooth-movement pain.The expression levels of TRPV1 mRNA and protein were determined by real-time PCR and western blot,respectively.Moreover,pain levels were assessed using the rat grimace scale (RGS).The role of TRPV1 in modulating tooth-movement pain was examined by injecting a TRPV1 antagonist into the trigeminal ganglia of rats.A lentivirus containing a TRPV1 shRNA sequence was constructed and transduced into the rats' trigeminal ganglia.The results showed that the expression levels of TRPV1 protein and mRNA were elevated following tooth-movement pain.Pain levels increased rapidly on the 1st day,peaked on the 3rd day and returned to baseline on the 14th day.The TRPV1 antagonist significantly reduced tooth-movement pain.The lentivirus containing a TRPV1 shRNA sequence was able to inhibit the expression of TRPV1 and relieved tooth-movement pain.In conclusion,TRPV1-based gene therapy may be a treatment strategy for the relief of orthodontic pain.
