Development of Pre-treatment Methods for Analysis of Different Oxidation States of Antimony Isotopes Based on Chromatographic Separation
10.19756/j.issn.0253-3820.241253
- VernacularTitle:基于色谱分离的不同价态锑同位素分析的前处理方法
- Author:
Shi HAN
1
;
Guang-Yi SUN
;
Xin-Yu LI
;
Xin-Bin FENG
Author Information
1. 中国科学院地球化学研究所,环境地球化学国家重点实验室,贵阳 550081;中国地质大学(武汉)地球科学学院,武汉 430074
- Keywords:
Antimony;
Antimony isotopes;
Speciation separation;
Analysis;
Multi-collector inductively coupled plasma mass spectrometer;
High-performance liquid chromatography
- From:
Chinese Journal of Analytical Chemistry
2024;52(10):1536-1543
- CountryChina
- Language:Chinese
-
Abstract:
Antimony is a toxic metalloid and exhibits different geochemical properties in different valence states,leading to distinct migration,transformation,and behavioral patterns in the environment.To further study the transfer and source-sink relationship of antimony and its compounds in the earth's surface ecological environment,it is very important to accurately determine the isotopic composition of antimony with different speciations.In this study,a pretreatment method for chromatographic separation and determination of different speciation's antimony isotopes in water samples was established by using multi-collector inductively coupled plasma mass spectrometry(MC-ICP-MS)to measure the isotopic composition of different speciation's antimony.This method was successfully applied to detection of natural rainwater and groundwater samples from the Qilong mining area.During the pretreatment,5 mmol/L ethylenediaminetetraacetic acid dipotassium salt(EDTA)and 2 mmol/L potassium hydrogen phthalate(KHP)(pH 4.5)were used as mobile phase,successfully separating trivalent antimony(Sb(Ⅲ))and pentavalent antimony(Sb(Ⅴ))from water samples using a Hamilton PRP-X100 anion exchange chromatographic column.A series of single-factor experiments identified the optimal separation and determination conditions as a column temperature of 40 ℃ and a high-performance liquid pump flow rate of 1.5 mL/min.The recoveries of Sb(Ⅲ)and Sb(Ⅴ)from the standard solutions of Sb(Ⅲ)and Sb(Ⅴ),and the mixed oxidation states solutions were 99％±1％,103％±1％,106％±1％,and 101％±1％,respectively.Moreover,the isotopic compositions of different speciation's antimony remained consistent before and after the chromatographic column treatment,and the reliability of the method was verified by detecting different oxidation state antimony in the natural rainwater samples.This research might provide important technical support for further studies on the geochemical cycling of antimony and its compounds in the ecological environment of the Earth's surface.
