High Throughput Organoid Culture and Its Research and Application Progress in Drug Screening
10.19756/j.issn.0253-3820.241340
- VernacularTitle:高通量类器官培养及其在药物筛选中的研究和应用进展
- Author:
Xin-Yu ZHENG
1
;
Jian-Zhang PAN
;
Qun FANG
Author Information
1. 浙江大学化学系,微分析系统研究所,杭州 310058
- Keywords:
Organoids;
Microfluidic;
High throughput;
Automation;
Drug screening;
Review
- From:
Chinese Journal of Analytical Chemistry
2024;52(10):1475-1486
- CountryChina
- Language:Chinese
-
Abstract:
Organoids are three-dimensional cell complexes formed by stem cells or tumor cells cultured and self-organized in vitro,which have higher simulation in morphology and function than the traditional two-dimensional cell drug screening model.However,the conventional organoid culture technology based on matrigel dome in culture dish or well plate has the problems of low throughput and time-consuming,which limits the application of high-throughput organoid drug screening.Therefore,a series of microfluidic high-throughput organoid culture and drug screening technologies have been developed.From the perspective of microfluidic technology,this paper reviews the current application of high-throughput organoid drug screening in three aspects:organoid culture,drug delivery and result detection.According to the different forms of organoid culture units,organoid culture techniques are divided into three categories:microfluidic droplets,microwells,and micropillars.According to the structure and working mode of drug delivery system,drug delivery system is divided into microchannel network and drug droplet array.According to the different forms of information obtained,high-throughput results detection methods can be divided into four categories:microplate reader,microscope,high-content imaging analyzer and chip sensor.Based on the above technologies,the application of typical organoid drug screening is introduced.Finally,the summary and prospect of organoid drug screening are presented.
