Tripod DNA Probe-based Electrochemiluminescent Biosensor for Detection of Exosome
10.19756/j.issn.0253-3820.241100
- VernacularTitle:基于三脚架DNA探针的电化学发光生物传感器检测外泌体
- Author:
Zhang-Jin HE
1
;
Xin JIN
;
Yu-Ying DU
;
Jie-Ning WU
;
Li-Ping LU
Author Information
1. 北京工业大学环境科学系,区域大气复合污染防治北京市重点实验室,北京 100124
- Keywords:
Exosomes;
Tripod DNA;
Aptamer;
Biosensors;
Electrochemiluminescence
- From:
Chinese Journal of Analytical Chemistry
2024;52(9):1328-1336
- CountryChina
- Language:Chinese
-
Abstract:
The detection of exosomes is of great significance for early diagnosis and treatment of cancer.In this study,based on the π-π interaction of the adenine bases of DNA with graphene,triple-stranded DNA(tsDNA)was assembled on the surface of electrochemically reduced graphene oxide(ERGO)to obtain a tripod DNA probe.The exosomes was specifically captured by exosome membrane protein(CD63)aptamer(apt)sequence at the top of tsDNA,and the introduction of hemin effectively enhanced the signal intensity of the Luminol-hydrogen peroxide(H2O2)electrochemiluminescence(ECL)system.The ECL intensity of the biosensor showed a good linear relationship with logarithm of exosome concentration in the concnetrtion range of 70-1.4×106 particles/μL,and the detection limit was 30 particles/μL(S/N=3).This tripod-structured DNA probe facilitated the capture and detection of larger targets,and was expected to be applied to the analysis of actual clinical samples.
