Investigation of Adductive Characteristics of Sulfur Mustards with Active Thiols
10.19756/j.issn.0253-3820.231449
- VernacularTitle:硫芥与活性硫醇化合物加合特性的分析研究
- Author:
Meng-Yao ZHANG
1
;
Jin-Long CAI
;
Meng-Qiang GONG
;
Bin XU
;
Jian-Feng WU
;
Hai-Xia WU
;
Jian-Wei XIE
Author Information
1. 河北科技大学化学与制药工程学院,石家庄 050091;军事医学研究院国家安全特需药品全国重点实验室,北京 100850
- Keywords:
Sulfur mustards;
Adducts;
Glutathione;
Cysteine;
N-Acetylcysteine
- From:
Chinese Journal of Analytical Chemistry
2024;52(7):995-1003,中插30-中插34
- CountryChina
- Language:Chinese
-
Abstract:
An analytical method based on ultra high performance liquid chromatography-high resolution tandem mass spectrometry(UHPLC-HRMS/MS)and high performance liquid chromatography-triple quadrupole mass spectrometry(HPLC-TQ MS)was established to reveal the characteristics of various sulfur mustard analogs with different active thiol molecules in CWC Schedule 1.A.04.Firstly,the toxic agents were prepared by micro-directed synthesis,and then the differences of the reactivity and abundance of formed adducts between different sulfur mustards and glutathione(GSH),cysteine(Cys)and N-acetylcysteine(NAC)in incubation solution,plasma and cell were investigated,respectively.The results indicated that all target sulfur mustards could react with three kinds of thiol molecules.The content of Cys and sulfur mustard adducts in plasma was higher than that of GSH and sulfur mustard adducts,while NAC and sulfur mustard adducts might have fewer types of adducts due to low content or poor mass spectrometry response.Additionally,the content of GSH and sulfur mustard adducts in exposed cells was higher than that of Cys,which should be due to the significant difference in the content of thiol molecules in plasma and cells.
