Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Analysis and Imaging of Porcine Brain Tissue Section Using Precoated Nanocarbon-Sodium Dodecyl Sulfate Composite Matrix
10.19756/j.issn.0253-3820.241085
- VernacularTitle:基于预涂纳米碳-十二烷基硫酸钠复合基质的基质辅助激光解吸电离质谱分析与猪脑组织切片质谱成像
- Author:
Si-Jia ZHUANG
1
;
Yu-Lin SHEN
;
Fan YANG
;
Can GONG
;
Xu XU
Author Information
1. 上海应用技术大学化学与环境工程学院,上海 201418
- Keywords:
Matrix assisted laser desorption ionization mass spectrometry;
Nanocarbon matrix;
Sodium dodecyl sulfate;
Tissue section;
Mass spectrometry imaging
- From:
Chinese Journal of Analytical Chemistry
2024;52(7):985-994,中插22-中插29
- CountryChina
- Language:Chinese
-
Abstract:
Nanomaterial matrices have unique advantages in small molecule analysis by matrix-assisted laser desorption ionization mass spectrometry(MALDI-MS).On the basis of comparing different surfactants,a method for pre-coating nanocarbon sodium dodecyl sulfate(SDS)composite matrix was established,which could alleviate the issue of aggregation of nanocarbon particles as a matrix.The experimental results showed that the nanocarbon-SDS composite pre-coated matrix effectively suppressed the background ion peak of the nano matrix,significantly improved the MS peak intensity and signal-to-noise ratio of the tested substance.Under the optimal conditions,cholesterol,1,2-dipalmitoyl-sn-glyceryl-3-phosphorylcholine(DPPC),and triglyceride standards were analyzed,with intra-point repeatability(RSD)of MS peak intensity≤5%and inter-point repeatability≤7%.As to measurement of cyclic adenosine monophosphate and DPPC samples in the range of 0.05?1.0 mg/mL,linear correlation coefficient(R2)between peak intensity and concentration was greater than 0.993,indicating good quantitative analysis potential.The nanocarbon-SDS composite pre-coating matrix was used for MALDI-MS imaging of pig brain tissue,and the differences in component distribution between pig brain white matter and gray matter were observed,demonstrating the potential of nanocarbon-SDS composite pre-coating matrix for MALDI-MS imaging.
