Development of Bacterial Drug Susceptibility Testing Method Based on Antibiotic Composite Fiber Membrane
10.19756/j.issn.0253-3820.231251
- VernacularTitle:抗生素复合纤维膜用于抗菌药物敏感性检测
- Author:
Yin-Li GUO
1
;
Chen-Yu ZHAO
;
Luan-Yue JI
;
Zhen-Tan LU
Author Information
1. 武汉纺织大学,纺织纤维及制品教育部重点实验室,武汉 430200;智能纺织材料与应用湖北省国际科技合作基地,武汉 430200;武汉纺织大学材料科学与工程学院,武汉 430200
- Keywords:
Antibiotic composite fiber membrane;
Colorimetric method;
Antimicrobial drug;
Susceptibility testing;
Rapid detection
- From:
Chinese Journal of Analytical Chemistry
2024;52(5):725-734,中插14-中插17
- CountryChina
- Language:Chinese
-
Abstract:
Efficient bacterial drug susceptibility test can help precise guidance on using antimicrobial and improve the efficiency of development of antibiotics.Traditional antimicrobial susceptibility testing(AST)methods are simple,reliable and specific,but suffer from drawbacks of time-consuming.The CCK8 reagent can be reduced by the dehydrogenase in the bacterial cells to form a water-soluble orange-yellow formazan,and the concentration of viable bacteria is proportional to the absorbance of formazan at 450 nm(OD450 nm).In this work,based on the principle of CCK8 colorimetry,the antibiotic loaded composite fiber membrane was used as the culture matrix for AST.Simple,rapid and high-throughput AST was realized by the difference in OD450 nm,which reflected the differences in viable bacteria quantity during bacterial growth.The total detection time was less than 8 h.The potential application of the method was evaluated using S.aureus,P.aeruginosa in wound simulation fluid and E.coli in artificial urine as simulated actual samples.The results of this method were in consistent with the disk diffusion test.This method provided a new idea for AST,which was helpful for the guidance of clinical medication and the development of antibiotics.
