Advances in Quantification and Site Stoichiometry Analysis Methods for Phosphorylated Proteins
10.19756/j.issn.0253-3820.231219
- VernacularTitle:磷酸化蛋白质的定量与化学计量分析方法的研究进展
- Author:
Yuan LIU
1
;
Rui ZHAI
;
Fan WU
;
Zhan-Ying CHU
;
Yang ZHAO
;
Xin-Hua DAI
;
Xiang FANG
;
Xiao-Ping YU
Author Information
1. 中国计量大学生命科学学院,浙江省生物计量及检验检疫技术重点实验室,杭州 310018;中国计量科学研究院前沿计量科学中心,国家市场监管技术创新中心(质谱),北京 100029
- Keywords:
Phosphorylated protein;
Enrichment process;
Mass spectrometry;
Quantitative approach;
Stoichiometry;
Review
- From:
Chinese Journal of Analytical Chemistry
2024;52(5):609-623
- CountryChina
- Language:Chinese
-
Abstract:
The post-translational modification of proteins is a key mechanism that imparts physiological functions to proteins,among which reversible phosphorylation modifications play a pivotal role in many biological processes.Aberrant changes in phosphorylation are often closely associated with various major disease processes.In recent years,with the aid of proteomic technologies and methods,high-throughput,high-precision qualitative and quantitative approaches for phosphorylated proteins have rapidly advanced.This article reviews the research progress of phosphorylated protein quantification and chemical proteomics analysis methods based on the"bottom-up"strategy,including phosphopeptide enrichment methods,mass spectrometry fragmentation methods,quantification analysis methods and phosphorylation site stoichiometry,and discusses the development trend of quantification and stoichiometric analysis methods for phosphorylated proteins.
