Oxygen-glucose deprivation preconditioning alleviates oxygen-glucose deprivation/reperfusion injury in PC12 cells by protecting mitochondria
10.16016/j.2097-0927.202311019
- VernacularTitle:氧糖剥离预处理通过保护线粒体减轻PC12细胞氧糖剥离/再灌注损伤
- Author:
Li HUANG
1
;
Na XU
;
Chaoxian YANG
Author Information
1. 400037 重庆,陆军军医大学(第三军医大学)第二附属医院麻醉科;646000 四川泸州,西南医科大学基础医学院解剖教研室
- Keywords:
oxygen-glucose deprivation preconditioning;
oxygen-glucose deprivation/reperfusion;
mitochondria;
PC12 cells
- From:
Journal of Army Medical University
2024;46(15):1763-1771
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of oxygen-glucose deprivation preconditioning(OGDPC)on PC 12 cells damaged with oxygen-glucose deprivation/reperfusion(OGD/R)and its underlying mechanism.Methods PC 12 cells were randomly divided into Control,OGDPC,OGD/R and OGDPC+OGD/R groups.The morphology and quantity of mitochondria were observed by transmission electron microscopy(TEM)and Mito-tracker staining.The expression of OGDH,PDHA1,COX4 and HSP60 was detected by Western blotting.Mitochondrial membrane potential and ATP production were evaluated by JC-1 and ATP assay kit.TUNEL staining was used to assess cell apoptosis,and translocation of AIF and EnodoG was observed by immunofluorescence staining and TUNEL staining.Results Compared with the OGD/R group,the mitochondrial ultrastructure damage were alleviated,the expression levels of OGDH,PDHA1 and COX4 were enhanced(P＜0.05),that of HSP60 was decreased(P＜0.05),the mitochondrial membrane potential and ATP level were increased(P＜0.05),the cell apoptotic rate was reduced(P＜0.05),and AIF and EndoG were less aggregated to the TUNEL-positive nucleus in OGDPC+OGD/R group(P＜0.05).Conclusion OGDPC can alleviate the injury of PC12 cells caused by OGD/R,and the potential mechanism may be related to improving mitochondrial structure and function,and reducing cell apoptosis via mitochondrial pathway.
