Correlation between intestinal bacteria Phascolarctobacterium/Phascolarctobacterium faecium and metabolic disorder in patients with type 2 diabetes mellitus
10.16016/j.2097-0927.202310036
- VernacularTitle:肠道细菌Phascolarctobacterium/Phascolarctobacterium faecium与2型糖尿病中代谢紊乱的关联分析
- Author:
Lisha LI
1
;
Daqian XIONG
Author Information
1. 610072 成都,成都中医药大学附属医院检验科
- Keywords:
type 2 diabetes mellitus;
Phascolarctobacterium;
Phascolarctobacterium faecium;
multi-omics analysis
- From:
Journal of Army Medical University
2024;46(13):1561-1568
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the differential microbiota in patients with newly diagnosed type 2 diabetes mellitus(T2DM),and to preliminarily evaluate the importance of the differential core microbiota in the metabolic disorder.Methods A total of 30 T2DM patients who were treated at the Endocrinology Department of our hospital from September to November 2020,and 30 healthy individuals taking physical examination during the same period were enrolled and assigned into the T2DM group and the control group,respectively.Their blood and fecal samples were collected for the detection of glucose,insulin,and lipid indicators levels and for gut microbiota using 16SrRNA,respectively.Subsequently,bioinformatics analysis,Pearson correlation analysis,and other statistical methods were applied to analyze the differences in T2DM blood indicators and specific bacterial species between the 2 groups.RT-qPCR was used to verify the screening results of small RNA profiles.Then a multi-parameter receiver operating characteristic(ROC)curve model was constructed based on the results of correlation analysis including microbiome,lipid metabolome and miRNAs spectrum genomic detection data of early research.Results T2DM showed specific characteristics of intestinal microbiota,with Phascolarctobacterium and Phascolarctobacterium.faecium(P.faecium)showing significant differences before and after the onset of the disease.Pearson correlation analysis suggested that P.faecium was negatively correlated with high-density lipoprotein(HDL)(r=-0.78,P＜0.001),low-density lipoprotein(LDL)(r=-0.89,P＜0.001),and insulin(r=-0.71,P＜0.001).While,Phascolarctobacterium was negatively correlated with insulin(r=-0.67,P＜0.001)and body mass index(BMI)(r=-0.64,P＜0.001).The ROC curve model based on Phascolarctobacterium/P.faecium,α-linolenic acid,and miR-122-5p could accurately distinguish between T2DM patients and healthy individuals,with a value of area under the curve of 0.928(P=0.0001)and 0.934(P＜0.0001),which suggesting core values of 2 types of bacteria in the disease.Conclusion T2DM patients have specific gut microbiota distribution,in which Phascolarctobacterium and P.faecium are closely associated with metabolic disorders of the disease.The 2 types of bacteria can be regarded as core bacteria for T2DM.
