Impact of intrauterine infection and perinatal antibiotic treatment on neonatal early gut floras
10.3969/j.issn.1671-8348.2024.18.005
- VernacularTitle:宫内感染和围产期抗生素治疗对新生儿早期肠道菌群的影响
- Author:
Ligang ZHOU
1
;
Yan WU
;
Siwei YI
;
Jie SHEN
Author Information
1. 重庆医科大学附属妇女儿童医院儿科,重庆 401147
- Keywords:
neonate;
intrauterine infection;
chorioamnionitis;
antibiotics;
gut flora;
high-throughput sequencing
- From:
Chongqing Medicine
2024;53(18):2744-2749
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of intrauterine infection combined with perinatal antibi-otic treatment on early neonatal gut microbiota.Methods Forty-six full term neonates delivered and hospital-ized in the Pediatrics Department of Women and Children's Hospital of Chongqing Medical University from January to June 2022 were selected as the study subjects.They were divided into the observation group (hav-ing,n=22) and the control group (not having,n=24) according to whether or not the mothers having chorio-amnionitis.The fecal samples were collected from the subjects on 0-2 d and 5-7 d after birth.The high-throughput sequencing technology was applied to conduct the bacterial 16S rRNA gene sequencing analysis in the fecal samples.The diversity,classification and relative abundance of gut floras were compared.Results Compared with the control group,the antibiotics use rate in prenatal and neonatal period in the observation group was higher,the antibiotic use duration in the neonatal period was longer,and the differences were statistically sig-nificant (P<0.05).The Shannon index on 5-7 d after birth in the two groups was increased compared with that on 0-2 d,moreover the observation group was lower than the control group,and the differences were statistically significant (P<0.05).The two groups of gut floras were mainly composed of Proteobacteria,Fir-micutes,Bacteroidetes,and Actinobacteria. Compared with the control group,the relative abundance of Pro-teobacteria on 5-7 d after birth in the observation group was higher,and the relative abundance of Actinobac-teria was lower,the differences were statistical significant (P<0.05). The two groups of gut floras were mainly composed of Bifidobacterium,Enterococcus,Streptococcus,Klebsiella,Bacteroidetes,and Escherichia/Shigella genera.Compared with the control group,the relative abundance of Bifidobacterium,Streptococcus and Bacteroides on 5-7 d after birth in the observation group was lower,the relative abundance of Klebsiella,and Escherichia/Shigella was higher,the differences were statistically significant (P<0.05).Conclusion In-trauterine infection and perinatal antibiotic treatment decrease the diversity of early neonatal gut flora,and the development of specific floras is delayed.
