Liposome-C-erbB2 antisense oligodoxynucleotides in human ovarian cancer cells
10.3760/j.issn:0366-6999.2001.07.016
- VernacularTitle:脂质体-C-erbB2反义寡核苷酸对卵巢癌细胞的作用
- Author:
Mei SHEN
1
;
Youji FENG
;
Baiqing GE
;
Zhijiang WU
;
Mingwei ZHU
Author Information
1. 上海医科大学附属妇产科医院
- Keywords:
proto-oncogene;
oligodeoxynucleotides;
antisense;
ovarian neoplasms
- From:
Chinese Medical Journal
2001;114(7):735-737
- CountryChina
- Language:Chinese
-
Abstract:
Abstract:Objective To explore the effects of liposome-C-erbB2 antisense phosphorothioate oligodeoxynucleotides (S-ODNs) on C-erbB2 proto-oncogene expression and cell proliferation in human ovarian cancer cells.Methods The effects of liposome-C-erbB2 S-ODNs on C-erbB2 protein expression, cell cycle and cell proliferation in human ovarian cancer cells were studied by means of flow cytometry and 3H-thymidine incorporation.Results Liposome-C-erbB2 S-ODNs can specifically reduce C-erbB2 protein expression in human ovarian cancer cells, accompanied by a 30% inhibition of cell proliferation. The effectiveness of liposome-C-erbB2 S-ODNs on the expression of C-erbB2 was about 40 times higher than that of C-erbB2 S-ODNs.Conclusions The data suggest that antisense therapy might be a useful method of gene therapy in ovarian cancer. The effectiveness of C-erbB2 S-ODNs could be greatly increased by adsorption of S-ODNs by liposomes.
