Detection of Fas/APO-1 in six human urogenital malignant cell lines with flow cytometry
- Author:
Hongjun LI
1
;
Lizhang YU
;
Yinglu GUO
;
Yi DING
;
Libo LIU
Author Information
1. The First Hospital of Beijing Medical University, Institute of Urology, Beijing Medical University
- From:
Chinese Medical Journal
1998;111(5):408-411
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of Fas/APO-1 in urogenital tumor cell lines.Methods With direct immunofluorescence, the expression of Fas/APO-1 in six urogenital malignant cell lines and one primary in vitro cultured normal renal fibroblast was detected by flow cytometry. Results Expression of Fas/APO-1 was detected in all six urogenital tumor cell lines, but with limited positive cell percentage and relatively lower fluorescence intensity, compared with expression of Fas/APO-1 in normal control of primary in vitro cultured renal fibroblast.Conclusions Lower expression of Fas/APO-1 in urogenital malignant cell lines than that in normal cells might be the reason for occurrence and progression of urogenital malignant tumors.
