Targeted diagnosis and treatment of superficial bladder cancer with monoclonal antibody BDI-1
- Author:
Lizhang YU
1
;
Fangliu GU
;
Chunli ZHANG
;
Shusheng XIE
;
Yinglu GUO
Author Information
1. 北京医科大学附属第一医院
- From:
Chinese Medical Journal
1998;111(5):404-407
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the application of monoclonal antibody (McAb) to targeted treatment of bladder carcinoma through a series of in vitro and in vivo studies carried out in animal model and patients with bladder carcinoma.Methods Monoclonal antibody BDI-1 against bladder carcinoma was prepared by the lymphocyte hybridoma technique. McAb was conjugated with 99m Tc by direct reduction method. Momodin (MD) was covalently linked to McAb by SPDP method. Radioimmunoimaging of nude mice xenografts and patients with bladder carcinoma were performed with BDI-1-99mTc conjugates. An immunotoxin (BDI-1-MD) was inducted via a catheter into the bladder. Targeted treatment with BDI-1-MD was carried out in 18 patients.Results This study showed the specificity of McAb, and clear imaging of nude mice bearing xenografts. Distribution analysis of 99m Tc-BDI-1 in nude mice showed the highest value of T/NT in bladder tumor. Targeted diagnosis and treatment for patients by intravesical administration are very safe and effective.Conclusion The bladder cancer seems an ideal model for diagnostic and therapeutic approaches using regional administration of McAb conjugates via a catheter direct into the bladder.
