11beta-hydroxysteroid dehydrogenase type 2 enzyme activity effect after exposures phthalate esters in maternal
10.3760/cma.j.issn.0253-9624.2014.09.011
- VernacularTitle:邻苯二甲酸酯暴露对产妇11β-羟基类固醇脱氢酶活性的影响
- Author:
Xiaoya HU
1
;
Yan ZHAO
;
Beitao CHEN
;
Yuan LIANG
;
Luxi LI
;
Changming XIE
;
Yunhui ZHANG
;
Zhenlang LIN
;
Ailan XIE
;
Shangqin CHEN
Author Information
1. 325027温州医科大学附属第二医院育英儿童医院
- Keywords:
Fetal growth retardation;
Tetrahydrocortisol;
Phthalate esters;
Intrauterine retarded;
11β-hydroxy steroid dehydrogenase
- From:
Chinese Journal of Preventive Medicine
2014;(9):800-804
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the association between phthalate esters ( PAEs ) metabolites in maternal urine and 11beta-hydroxysteroid dehydrogenase type 2(11β-HSD2 ) enzyme activity, explore the possible mechanism of PAEs effect on fetal development.Methods All of 33 cases of intrauterine growth retardation ( IUGR) newborn were selected by random sampling in 2012.And 33 cases of normal control newborn were enrolled , use high performance liquid chromatography-tandem mass spectrometry method was used to detect 4 kinds of phthalate esters ( PAEs) metabolites in maternal urine:mono-n-butyl phthalate ester (MBP), mono (2-ethylhexyl) phthalate (MEHP), mono (2-ethyl-5-hydroxyhexyl) phthalate(MEHHP), mono ( 2-ethyl-5-oxohexyl ) phthalate ( MEOHP ) and three kinds of cortisol corticosterone metabolites , tetrahydrocortisol (THF), allo-tetrahydrocortisol (allo-THF), tetrahydrocortisone (THE), and analyze the association between phthalate esters ( PAEs) metabolites in maternal urine and 11β-HSD2 enzyme activity.Results MBP,MEHP,MEHHP,MEOHP metabolites can be detected in 98%(65 cases),89%(59 cases), 91%(60 cases),91%(60 cases)of all 66 maternal urine samples, respectively.The median concentrations of test material in case group were 31.20 ng/ml for MBP, 24.61 ng/ml for MEHHP, 11.72 ng/ml for MEOHP and 48.67 ng/ml for SumDEHP which were significantly higher than those of the control group ( were 17.32,12.03,5.68 and 28.64 ng/ml); 11β-HSD2 activity in case group (( THF +allo-THF)/THE =(0.79 ±0.09) ng/ml) was significantly lower than that of the control group (( THF+allo-THF)/THE=(0.58 ±0.04) ng/ml);PAEs metabolites MBP(β′=1.12),MEHHP(β′=1.14),MEOHP(β′=1.10), SumDEHP (β′=1.08 ) in baby boy mother′s urine was reversely correlated to 11β-HSD2 activity.Conclusions PAEs could affect fetal development by inhibit 11β-HSD2 activity.
