Detecting Bacterial Growth in Continuous Ambulatory Peritoneal Dialysis Effluent Using Two Culture Methods.
10.3904/kjim.2010.25.1.82
- Author:
Se Hee YOON
1
;
Nak Won CHOI
;
Sung Ro YUN
Author Information
1. Department of Internal Medicine, Konyang University College of Medicine, Daejeon, Korea. sehei@hanmail.net
- Publication Type:Original Article ; Comparative Study
- Keywords:
Culture media;
Peritoneal dialysis;
Peritonitis;
Sensitivity and specificity
- MeSH:
Culture Media;
Dialysis Solutions;
Gram-Negative Bacterial Infections/*diagnosis/microbiology;
Gram-Positive Bacterial Infections/*diagnosis/microbiology;
Humans;
Kidney Failure, Chronic/*therapy;
Microbiological Techniques/*methods;
Peritoneal Dialysis, Continuous Ambulatory/*adverse effects;
Peritonitis/*diagnosis/microbiology;
Sensitivity and Specificity
- From:The Korean Journal of Internal Medicine
2010;25(1):82-85
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: The aim of this study was to evaluate the peritonitis-causing bacteria detected in peritoneal fluid using a blood culture bottle in patients undergoing continuous ambulatory peritoneal dialysis (CAPD). METHODS: One-hundred and eleven dialysates from 43 patients suspected of peritonitis related to CAPD were retrospectively evaluated between May 2000 and February 2008. In all cases, 5 to 10 mL of dialysate was inoculated into a pair of BacT/Alert blood culture bottles, and 50 mL of centrifuged dialysate was simultaneously inoculated into a solid culture media for conventional culture. The results were compared to those of the conventional culture method. Isolated microorganisms were compared between the two methods. RESULTS: The blood culture method was positive in 78.6% (88 / 112) of dialysate specimens and the conventional culture method in 50% (56 / 112, p < 0.001). CONCLUSIONS: The blood culture method using the BacT/Alert system is useful for culturing dialysates and improves the positive culture rate in patients with suspected peritonitis compared to the conventional culture method.
