Expression of prostaglandin transporter in colorectal cancer tissues and its relationship with clinicopathological features
10.3760/cma.j.issn.1671-0274.2015.03.017
- VernacularTitle:前列腺素转运蛋白在结直肠癌组织中的表达及其与临床病理特征的关系
- Author:
Shanliang SHANG
1
;
Xiujun LIAO
;
Zhong SHEN
;
Jianming QIU
;
Shuxian SHAO
;
Lie DING
;
Dong WANG
;
Guangen YANG
;
Yanxiang ZHANG
Author Information
1. 310009,安徽医科大学附属杭州市第三人民医院肛肠外科
- Keywords:
Colorectal neoplasms;
Prostaglandin transporter;
Prostaglandin E2;
Clinicopathological features
- From:
Chinese Journal of Gastrointestinal Surgery
2015;(3):277-281
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of prostaglandin transporter (PGT) in colorectal cancer (CRC) tissues and its relationship with clinicopathological features. Methods The mRNA and protein levels of PGT were determined by real-time PCR , Western blot and immunohistochemical methods in cancer tissues and adjacent normal tissue from 80 patients with colorectal cancer and their relationship with clinicopathological features was analyzed. Results Compared with the adjacent normal tissue of colorectal cancer , the PGT mRNA relative expression (0.57 ±0.33 vs. 2.33 ±1.20) and the PGT protein expression in cancer tissues decreased significantly [PGT/GAPDH 0.45 ±0.16 vs. 0.78 ±0.23, integral A 718.7 ±359.4 vs. 10412.0 ±6423.3, average A 0.03 ±0.01 vs. 0.12 ±0.09, all P<0.01]. Lower mRNA and protein expressions of PGT in colorectal cancer were associated with depth of invasion T3 to T4 and TNM stage Ⅲ to Ⅳ (P<0.01), while not associated with gender, age, tumor location and differentiation degree (all P>0.05). Conclusion Expression levels of PGT mRNA and protein in colorectal cancer tissue are significantly down-regulation. PGT expression is associated with invasion depth and late stages.
