Experimental study of adipose-derived mesenchymal stem cells in the treatment of Crohn′s disease
10.3760/cma.j.issn.1671-0274.2015.01.015
- VernacularTitle:脂肪来源间充质干细胞治疗克罗恩病的实验研究
- Author:
Minghao XIE
1
;
Xiaosheng HE
;
Jinling ZHU
;
Zhen HE
;
Xiaowen HE
;
Ping LAN
;
Lei LIAN
Author Information
1. 中山大学附属第六医院结直肠外科
- Keywords:
Adipose-derived mesenchymal stem cells;
Crohn′s disease;
Inflammatory cytokines;
Animal model,mouse
- From:
Chinese Journal of Gastrointestinal Surgery
2015;(1):58-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the efficacy of adipose-derived mesenchymal stem cells (ADMSCs) in a murine model of inflammatory bowel disease, and its potential mechanism. Methods Murine colitis mouse model of Crohn′s disease (CD) was created by trinitrobenzene sulfonic acid (TNBS)-induced colitis. Seventy-five 6-8 weeks female BALB/c mice were randomly divided into 3 groups: control group, TNBS group and ADMSC group. To verify the therapeutic effect of ADMSC, real-time PCR and immunohistochemical staining were performed to measure inflammatory cytokines levels in colon tissues. The 10-day survival statuses were recorded after the infusion of ADMSCs. Results Intraperitoneal injection of ADMSCs alleviated the clinical and histopathologic severity of intestinal inflammation, and increased survival(60% vs. 30%, P<0.05) in the TNBS-induced mouse model of CD. Compared with TNBS group, proinflammatory cytokines, including TNF-α, IL-12 and VEGF of ADMSC group were significantly reduced, with significant increase of IL-10 expression. Conclusion ADMSCs can effectively repair the injury of colonitis through down-regulation of proinflammatory cytokines TNF-α, IL-12 and VEGF expression, and up-regulation of anti-inflammatory cytokine IL-10 expression, which may be a potential new alternative of cell-based therapy for CD.
