Preparation method of acute myocardial infarction model induced by isoproterenol in mice
- VernacularTitle:异丙肾上腺素诱导小鼠急性心肌梗死模型制备方法研究
- Author:
Ming QIAN
1
;
Long CHENG
;
Yuan XIONG
;
Hui YANG
;
Li JI
Author Information
- Keywords: acute myocardial infarction; isoproterenol; creatine kinase; creatine kinase isoenzyme; cardiac troponin T; lactate dehydrogenase
- From: Journal of Clinical Medicine in Practice 2023;27(23):6-11
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effects of isoproterenol administration on myocardial in-jury and establish acute myocardial infarction(AMI)model in mice.Methods Mice were injected subcutaneously with isoproterenol(150 mg/kg),and blood samples were taken at different time points.The contents of AMI related indexes[creatine kinase(CK),creatine kinase isoenzyme(CK-MB),cardiac troponin T(cTnT),lactate dehydrogenase(LDH),alanine aminotransferase(ALT),aspar-tate aminotransferase(AST),interleukin 6(IL-6),procalcitonin(PCT)and C-reactive protein(CRP)]in serum were detected.The pathological changes of cardiac tissue were observed by hematoxy-lin-eosin staining.Results The serum levels of CK,CK-MB,LDH,cTnT,ALT and AST were signifi-cantly increased after isoproterenol model(P＜0.05,P＜0.01);after two consecutive administrations of isoproterenol,the serum AMI-related indexes increased significantly,and the relevant indexes reached the highest concentration 16 h after administration(P＜0.05,P＜0.01).Hematoxylin-eosin staining results showed that pathological changes occurred in myocardial tissue.Conclusion Subcutaneous injection of isoproterenol induces AMI injury.The AMI injury at 16 h after two consecutive injections is the most serious,which lays a foundation for clinical drug research and innovative drug development of AMI.
