Expression and significance of claudin-4 in colon mucosa of mice with inflammatory bowel disease

VernacularTitle:Claudin-4在炎症性肠病小鼠结肠黏膜中的表达及意义
Author: Guangrong HENG ¹ ; Yanbing DING ; Guotao LU
Author Information

1. 扬州大学附属医院消化科
Keywords: claudin-4; Dextran sulfate sodium; inflammatory bowel disease
From: Journal of Clinical Medicine in Practice 2017;21(5):11-14
CountryChina
Language:Chinese
Abstract: Objective To observe the claudin-4 expression in inflammatory bowel disease mice models which induced by dextran sulfate sodium (DSS).Methods Fourteen mice were established inflammatory bowel disease models induced by 7-day administration of 2.5％ DSS in water.The other six mice were fed with water only as control group.Pathological changes in colon mucosa were observed on the eighth day.Immunohistochemistry and western blot were used to measure the protein expression of claudin-4 in colon mucosa.Results IBD model was successfully established according to weight and pathological changes.The claudin-4 expression in the model group mice decreased significantly compared with control group and the difference was statistically significant(P ＜0.05).Conclusion Claudin-4 expression level decreased in colon mucosa of IBD mice.The change of the claudin-4 expression may play an important role in IBD development and progression.