Determination of Perchloroethylene in blood by headspace gas chromatography-mass spectrometry
10.3760/cma.j.cn121094-20221026-00514
- VernacularTitle:全血中四氯乙烯测定的自动顶空气相色谱-质谱法
- Author:
Jie FENG
1
;
Wubin LIU
;
Yan LI
;
Chunguang DING
Author Information
1. 国家卫生健康委职业安全卫生研究中心（国家卫生健康委煤炭工业职业医学研究中心），北京 102308
- Keywords:
Perchloroethylene (PCE);
Blood sample;
Gas chromatography-mass spectrometry;
Headspace analysis
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2024;42(1):42-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for determination of Perchloroethylene (PCE) in blood by headspace gas chromatography-mass spectrometry (HS/GC-MS) .Methods:From Dctober to December 2021, A total of 3 mL blood samples were taken into a 10 mL headspace bottle, after heated at 60 ℃ for 30 mins, PCE in the top air was separated by VF-WAXms capillary column and detected by GC-MS. The retention time and external standard method were used for qualitative and quantitative analysis of PCE in samples, respectively.Results:There was good linear relationship in the range of 5.09-200.17 μg/L. The linear correlation coefficient was 0.9993.The detection limit was 0.21 μg/L and the lower limit of quantitation was 0.70 μg/L. The recovery rates of samples with different concentrations were 95.3%-103.8%. The intra-batch relative standard deviations ( RSD) were 3.2%-4.6%, and inter-batch RSD was 4.0%-6.1%. The samples can be stored at 4 ℃ for three days and at -20 ℃ for seven days. Conclusion:This method is proved to be simple, practical and highly sensitive, which is suitable for the determination of PCE in blood.
