The effects of P-glycoprotein expression induced by ulinastatin on HK-2 cells damage induced by paraquat
10.3760/cma.j.issn.1001-9391.2016.11.002
- VernacularTitle:乌司他丁上调P糖蛋白表达对百草枯诱导HK-2细胞损伤的影响
- Author:
Xingrong SHE
1
;
Xin TIAN
;
Xingken FAN
;
Guangliang HONG
;
Guangju ZHAO
;
Mengfang LI
;
Zhongqiu LU
Author Information
1. 443000 宜昌,三峡大学第一临床医学院;温州医科大学附属第一医院急诊医学中心
- Keywords:
Paraquat;
Ulinastatin;
HK-2;
P-glycoprotein
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2016;34(11):805-809
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of P-glycoprotein up-regulated by ulinastatin (UTI) on HK-2 cells during paraquat (PQ)-induced injury and its underlying mechanisms.Methods The research was divided into two parts.The first part of the research was divided into normal control group,PQ group,UTI+PQ group,UTI control group.The second part of the research was divided into negative virus group (including control group,PQ group,PQU+TI group,UTI group) and P-gp siRNA group (including control group,PQ group,PQU+TI group,UTI group).Negative virus group:the cells were transfected into the blank virus;siRNA P-gp group:the cells were transfected with P-gp siRNA virus.HK-2 cells were routinely cultured.After 800 μmol/L PQ treatment,the changes of P-gp protein levels in the HK-2 cells were determined by Western-blot (WB).Then,transfected lentivirus bringing P-gp silent gene,the cell viability was determined by CCK-8 assay,the expression of P-gp in the cells after transfection was detected by WB and the concentration of PQ in HK-2 cells were measured by high performance liquid chromatography (HPLC).Results Compared with the normal control group,the P-gp expression of PQ group had no significantly changes (P＞0.05).Compared with the PQ group,the P-gp expression of UTI +PQ group significantly increased (P＞0.05).Compared with the corresponding control siRNA group,the P-gp siRNA group had no significantly changes in cell viability (P＞ 0.05).and significantly decreased in P-gp expression.Compared with the corresponding control siRNA group,the P-gp siRNA group had no significantly changes in PQ concentration in HK-2 cell (P＞0.05),but compared with P-gp siRNA PQ group,the PQ concentration of P-gp siRNA PQ+UTI group significantly decrease(P＜0.05).Conclusion UTI significantly reduced the accumulation of PQ in HK-2 cells and increased the viability of HK-2 cells in vitro may be not by increased P-gp activity.UTI could significantly reduce HK-2 cell injury induced by PQ in vitro and improve the survival rate of HK-2 cells.It may not be related to the up regulation of P-gp expression.
