Bioinformatics analysis and validation of the interaction between PML protein and TAB1 protein
10.12122/j.issn.1673-4254.2024.01.21
- VernacularTitle:早幼粒细胞白血病蛋白与TAK1结合蛋白相互作用的生物信息学分析与验证
- Author:
Jiacong CHENG
1
;
Zhihui LI
;
Yao LIU
;
Cheng LI
;
Xin HUANG
;
Yinxin TIAN
;
Fubing SHEN
Author Information
1. 成都医学院检验医学院,四川 成都 610500
- Keywords:
TAB1 protein;
PML protein;
bioinformatics;
protein-protein docking;
co-immunoprecipitation
- From:
Journal of Southern Medical University
2024;44(1):179-186
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the interaction between PML protein and TAB1 protein using bioinformatic approaches and experimentally verify the results.Methods Using Rosetta software,a 3D model of TAB1 protein was constructed through a comparative modeling approach;the secondary structure of PML protein was retrieved in the PDB database and its crystal structure and 3D structure were resolved.Zdock 3.0.2 software was used to perform protein-protein docking of PML and TAB1,and the best conformation was extracted for molecular structure analysis of the docking model.The interaction between the two proteins was detected using immunoprecipitation in α-MMC-treated M1 inflammatory macrophages.Results When 6IMQ of PML was used as the docking site,PML protein formed 3 salt bridges,6 hydrogen bonds and 6 hydrophobic interactions with TAB1 proteins;when 5YUF of PML was used as the docking site,PML protein formed 1 hydrogen bond,3 electrostatic interactions and 9 hydrophobic interactions with TAB1 proteins,and both of the docking modes formed good molecular docking and interactions.In the M1 inflammatory macrophages treated with α-MMC for 4 h,positive protein bands of PML and TAB1 were detected in the cell lysates in PML-IP group.Conclusion PML protein can interact strongly with TAB1 protein.
