RNA interference of HERC4 inhibits proliferation, apoptosis and migration of cervical cancer Hela cells
10.3969/j.issn.1673-4254.2017.02.15
- VernacularTitle:沉默HERC4表达可抑制宫颈癌细胞的增殖、凋亡和迁移
- Author:
Min WEI
1
;
Yanling ZHANG
;
Lan CHEN
;
Cuixia CAI
;
Handuo WANG
Author Information
1. 深圳市南山区妇幼保健院检验科
- Keywords:
HERC4;
small interfering RNA;
Hela cells;
proliferation;
apoptosis;
migration
- From:
Journal of Southern Medical University
2017;37(2):232-237
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of silencing HERC4 on the proliferation,apoptosis,and migration of cervical cancer cell line Hela and the possible molecular mechanisms.Methods Three HERC4-specific small interfering RNAs (siRNAs) were transfected into Hela cells,and HERC4 expression in the cells was examined with Western blotting.CCK-8 assay,annexin V-FITC/PI assay,and wound healing assay were used to assess the effect of HERC4 silencing on the proliferation,apoptosis and migration ability of Hela cells.The expression levels of cyclin D1 and Bcl-2 in the cells were detected using Western blotting.Results Transfection of siRNA-3 resulted in significantly decreased HERC4 protein expression (P＜0.01).HERC4 silencing by siRNA-3 markedly suppressed the proliferation and migration of Hela cells,increased the apoptosis rate (P＜0.01) and reduced the expression levels of cyclin D1 and Bcl-2 (P＜0.01).Conclusion Silencing of HERC4 efficiently inhibits the proliferation,migration,and invasion of Hela cells in vitro,and the underlying mechanisms may involve the down-regulation of cyclin D1 and Bcl-2.
