Biocompatibility of poly- L- lactic acid/Bioglass- guided bone regeneration membranes processed with oxygen plasma
- VernacularTitle:氧等离子处理PLLA/BG引导骨再生膜生物相容性
- Author:
Wei FANG
1
;
Shuguang ZENG
;
Wenfeng GAO
Author Information
1. 南方医科大学附属口腔医院//广东省口腔医院
- Keywords:
guided bone regeneration;
oxygen plasma;
poly-L-lactic acid;
Bioglass
- From:
Journal of Southern Medical University
2015;(4):567-572
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare and characterize a nano-scale fibrous hydrophilic poly-L-lactic acid/Bioglass (PLLA/BG) composite membrane and evaluate its biocompatibility as a composite membrane for guiding bone regeneration (GBR). Methods PLLA/BG-guided bone regeneration membrane was treated by oxygen plasma to improved its hydrophilicity. The growth of MG-63 osteoblasts on the membrane was observed using Hoechst fluorescence staining, and the biocompatibility of the membrane was evaluated by calculating the cells adhesion rate and proliferation rate. Osteogenesis of MG-63 cells was assessed by detecting alkaline phosphatase (ALP), and the formation of calcified nodules and cell morphology changes were observed using scanning electron microscope (SEM). Results The cell adhesion rates of PLLA/BG-guided bone regeneration membrane treated with oxygen plasma were (30.570 ± 0.96)%, (47.27 ± 0.78)%, and (66.78 ± 0.69)%at 1, 3, and 6 h, respectively, significantly higher than those on PLLA membrane and untreated PLLA/BG membrane (P<0.01). The cell proliferation rates on the 3 membranes increased with time, but highest on oxygen plasma-treated PLLA/BG membrane (P<0.01). Hoechst fluorescence staining revealed that oxygen plasma treatment of the PLLA/BG membrane promoted cell adhesion. The membranes with Bioglass promoted the matrix secretion of the osteoblasts. Under SEM, the formation of calcified nodules and spindle-shaped cell morphology were observed on oxygen plasma-treated PLLA/BG membrane. Conclusion Oxygen plasma-treated PLLA/BG composite membrane has good biocompatibility and can promote adhesion, proliferation and osteogenesis of the osteoblasts.
