Impact of topographic features of electrospun polymethylmethacrylate nanofibers on growth pattern of rat primary astrocytes
10.3969/j.issn.1673-4254.2014.11.03
- VernacularTitle:聚甲基丙烯酸甲酯静电纺丝纳米纤维对大鼠原代星形胶质细胞生长的影响
- Author:
Haijian XIA
1
;
Dan LIU
;
Dong ZHONG
;
Yongzhi XIA
;
Yi YAN
;
Wenyuan TANG
;
Xiaochuan SUN
Author Information
1. 重庆医科大学 附属第一医院神经外科
- Keywords:
electrospining;
polymethylmethacrylate;
astrocytes;
cell culture;
contact guidance;
spinal cord injury
- From:
Journal of Southern Medical University
2014;(11):1569-1573
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the impact of the topographic features of electrospun polymethylmethacrylate (PMMA) nanofibers on growth pattern of primary rat astrocytes. Methods Rat astrocytes were cultured on fabricated random and aligned electrospun nanofibers. Using PMMA film as the control, the cells were transfected with a lentivirus harboring GFP gene to reveal the cell growth pattern on different substrates. Results Both random and aligned electrospun PMMA nanofibers could support the growth of rat astrocytes, but the topographic features of the fibers significantly affected their growth pattern. On aligned nanofibers, astrocytes extended long cell processes along the direction of the substrate fibers, and on random fibers the astrocytes formed shorter processes. On merged images of GFP expressions and substrate fibers, the cell processes of the astrocytes were shown to adhere to and elongate along the fibers both on random and aligned nanofibers. Conclusion The topographic features of PMMA nanofibers can significantly influence the growth pattern of primary rat astrocytes. Aligned electrospun nanofibers has the potential to serve as scaffold material for reducing glia scar formation after spinal cord injury.
