Effect of spermatozoa from different sources on normal fertilization of oocytes and embryo quality and development in intracytoplasmic sperm injection cycles
10.3969/j.issn.1673-4254.2014.06.21
- VernacularTitle:ICSI中不同来源精子对卵母细胞受精、胚胎质量及发育潜能的影响
- Author:
Duo XIE
1
;
Zhuolin QIU
;
Chen LUO
;
Qingjun CHU
;
Song QUAN
Author Information
1. 南方医科大学南方医院生殖医学中心
- Keywords:
intracytoplasmic sperm injection;
sources of spermatozoa;
embryo quality;
blastocyst;
developmental potential,embryo
- From:
Journal of Southern Medical University
2014;(6):857-861
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the impact of spermatozoa from different sources on normal fertilization of oocytes, embryo quality and embryo developmental potential in intracytoplasmic sperm injection (ICSI) cycles. Methods A retrospective analysis was conducted among 197 patients undergoing ICSI cycles in our center. The patients were classified into 3 groups according to the sources of semen, namely ejaculated spermatozoa group (n=102), percutaneous epididymal sperm aspiration (PESA) group (n=68), and testicular sperm aspiration (TESA) group (n=27). The ejaculated spermatozoa group was further classified into oligoasthenoteratozoospermia (n=67) and cryptozoospermia (n=35) subgroups. The normal fertilization, high-quality embryo, implantation and clinical pregnancy rates were compared among the groups; the rate of high-quality blastocyst formation in in-vitro culture of non-top quality embryos was also observed. Results The patients with PESA showed significantly higher normal fertilization rate (75.6%) than those in oligoasthenoteratozoospermia (64.8%), cryptozoospermia (62.1%), and TESA (61.6%) groups (P<0.05). No significant differences were found in the high-quality embryo, implantation, and clinical pregnancy rates among the groups (P>0.05). The rate of high-quality blastocyst formation in the in-vitro culture of non-top quality embryos was also comparable among the groups (P>0.05). Conclusion Although spermatozoa obtained with by PESA is associated with a higher normal fertilization rate, the sources of spermatozoa do not significantly affect the embryonic quality and developmental potential in ICSI cycles.
