Small interfering RNA-mediated COX-2 gene silencing enhances chemosensitivity of KB/VCR cells by suppressing MDR-1 gene expression and P-glycoprotein activity
10.3969/j.issn.1673-4254.2014.05.26
- VernacularTitle:siRNA沉默COX-2基因与增强口腔癌耐药株KB/VCR细胞化疗敏感性的相关性
- Author:
Xianchao MO
1
;
Weizhong LI
Author Information
1. 南方医科大学南方医院口腔颌面外科
- Keywords:
oral neoplasm;
KB/VCR cells;
chemosensitivity;
P-glycoprotein;
cyclooxygenase-2;
small interfering RNA
- From:
Journal of Southern Medical University
2014;(5):718-722
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of small interfering RNA (siRNA)-mediated COX-2 gene silencing in enhancing the chemosensitivity of KB/VCR cell lines. Methods KB/VCR cells were trasnfected with COX-2 siRNA were examined for expressions of COX-2 and MDR-1 mRNAs with RT-PCR and for Rho-123 accumulation using flow cytometry. MTT assay was used to analyze the proliferation of the transfected KB/VCR cells. Results Compared with the negative and blank control groups, COX-2 siRNA transfection resulted in significant growth inhibition of KB/VCR cells exposed to vincristine (P<0.01), down-regulated the expressions of COX-2 and MDR-1 mRNAs, and obviously increased Rho-123 accumulation in KB/VCR cells. Conclusion COX-2 gene silencing can enhance the chemosensitivity of KB/VCR cells to vincristine, the mechanism of which may involve down-regulated MDR-1 gene expression and inhibition of P-glycoprotein activity.
