Human umbilical cord-derived mesenchymal stem cells inhibit proliferation but maintain survival of Jurkat leukemia cells in vitro by activating Notch signaling
10.3969/j.issn.1673-4254.2014.04.01
- VernacularTitle:人脐带间充质干细胞通过激活Notch信号通路促进白血病细胞株Jurkat的体外存活
- Author:
Yin YUAN
1
;
Danliang CHEN
;
Xuan CHEN
;
Hongwei SHAO
;
Shulin HUANG
Author Information
1. 广东药学院生命科学与生物制药学院/广东省生物技术候选药物研究重点实验室
- Keywords:
mesenchymal stem cells;
umbilical cord;
leukemia;
proliferation;
Notch signaling
- From:
Journal of Southern Medical University
2014;(4):441-447
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) on the proliferation and survival of Jurkat leukemia cells in vitro and explore the possible mechanism. Methods Jurkat leukemia cells were co-cultured with hUC-MSCs isolated from human umbilical cord tissues by plastic adherence at a ratio of 10∶1. The proliferation and survival of the co-cultured Jurkat cells, separated by immunomagnetic bead cell sorting on day 4, were evaluated by flow cytometry. Western blotting was performed to evaluate the activation of Notch signaling in the co-cultured Jurkat cells. Results Jurkat leukemia cells co-cultured with hUC-MSCs for 4 days showed a lowered proliferation rate and cell cycle arrest at G0/G1 phase with a reduction in the cell apoptotic rate. Notch signaling pathway was activated in the co-cultured Jurkat cells as evidenced by an increased cellular expression of HES-1. Conclusion Co-culture with hUC-MSCs can inhibit the proliferation of Jurkat leukemia cells in vitro and protect the cells from apoptosis by activating Notch signaling, indicating a potential shielding effect of MSCs on leukemia cells.
