Effect of interleukin-1β on expressions of activin A and its related factors in cultured endometrial stromal cells from patients with endometriosis
10.3969/j.issn.1673-4254.2014.03.25
- VernacularTitle:IL-1β诱导子宫内膜异位症间质细胞activin A及相关因子的表达
- Author:
Lei LI
1
;
Mubiao LIU
;
Fengcheng ZHU
;
Ying PAN
Author Information
1. 南方医科大学珠江医院妇产科
- Keywords:
endometriosis;
interleukin-1β;
activin A;
follistatin;
cripto
- From:
Journal of Southern Medical University
2014;(3):410-413
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of interleukin-1β(IL-1β) on the expressions activin A, follistatin, and cripto in cultured human endometrial stromal cells (HESCs) form patients with endometriosis. Methods Cultured HESCs were stimulated with 250, 500, and 750 pg/ml IL-1β, and the mRNA and protein expressions of activin A, follistatin, and cripto were assayed using real-time reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assay. Results IL-1βtreatment caused significant dose-dependent increments of the mRNA and protein expressions of activin A and follistatin and of the mRNA expression of cripto in cultured HESCs. Conclusion IL-1βcan affect the expressions of activin A, follistatin and cripto in HESCs from patients with endometriosis.
