Effects of peroxisome proliferator-activated receptorsγon the expression of insulin receptor substrate-4 gene in rat cortical neurons and mouse brain
- VernacularTitle:PPARγ在神经系统中能够促进IRS-4基因表达
- Author:
Hongyan ZHANG
1
;
Siying MENG
;
Lifang LIN
;
Qiaoqi WU
;
Riyang ZHOU
;
Xuemin WANG
Author Information
1. 南方医科大学基础医学院神经生物学教研室
- Keywords:
cortex;
rosiglitazone;
peroxisome proliferator-activated receptorsγ;
insulin receptor substrate-4
- From:
Journal of Southern Medical University
2013;(10):1463-1466
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of peroxisome proliferator-activated receptors γ (PPARγ) on insulin receptor substrate-4 (IRS-4) gene expression in the brain. Methods Primarily cultured cortical neurons from E17-18 Sprague Dawley rats, after 1 week of plating, were exposed to 10μmol/L PPARγagonist rosiglitazone for 0, 1, 4 or 24 h. Adult C57BL/6J mice or conditional brain PPARγ knock-out mice (B-PPARγ-KO, BKO) received an intraperitoneal injection of rosiglitazone in 10%DMSO at 12 mg/kg or injection of the same volume of saline containing 10%DMSO. The effect of rosiglitazone on the survival of the neurons was examined by MTT assay. The expression of IRS-4 mRNA was analyzed by real-time quantitative PCR. Results The survival of the cortical neurons showed no significant difference between the agonist groups and the control group. The expression of IRS-4 mRNA was significantly up-regulated in the cortical tissues and neurons of the agonist groups compared with the control groups (P<0.05), but in BKO mice without treatment, IRS-4 mRNA expression was significantly down-regulated (P<0.05). Conclusion PPARγcan enhance the expression of IRS-4 mRNA in the brain.
