Expression of signal recognition particle 14 in hepatocellular carcinoma and its relationship with disease progression and patient survival
10.3724/zdxbyxb-2024-0055
- VernacularTitle:信号识别颗粒14调控肝细胞癌进程并影响患者预后
- Author:
Huimin TIAN
1
;
Dongmei TANG
;
Meilin MA
;
Xianghui FU
Author Information
1. 四川大学华西医院内分泌代谢科 糖尿病与代谢研究室,四川 成都 610041
- Keywords:
Hepatocellular carcinoma;
Nonalcoholic fatty liver disease;
Signal recognition particle 14;
G protein-coupled receptor class C group 5 member A;
Cell proliferation;
Cell migration;
Prognosis
- From:
Journal of Zhejiang University. Medical sciences
2024;53(4):460-471
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of signal recognition particle 14(SRP14)in hepatocellular carcinoma(HCC)and its clinical significance.Methods:The data of SRP14 expression in HCC were obtained from bioinformatics study,and from investigation with quantitative reverse transcription polymerase chain reaction(qRT-PCR),immunohistochemical staining and Western blotting in clinical samples.The Kaplan-Meier analysis was used to determine the associations between SRP14 mRNA expression and the overall survival,progression-free survival,and disease-specific survival of HCC patients.The effect of SRP14 on the proliferation and migration of HCC cells were determined by EdU staining,MTS,Transwell and wound-healing assays.The potential mechanism for SRP14 regulating HCC was explored through Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO)enrichment analysis as well as qRT-PCR.Results:According to the data from GSE14520,TNMplot database and clinical samples,compared with paired tumor-adjacent tissues,non-paired tumor-adjacent tissues and normal tissues,the mRNA expression of SPR14 in HCC tissues was upregulated(all P<0.05).In clinical samples,compared with paired tumor-adjacent tissues,the protein expression of SPR14 in HCC tissues was increased(P<0.05).The increased mRNA expression of SRP14 was associated with good overall survival,progression-free survival,and disease-specific survival in HCC patients.SRP14 inhibited the proliferation and migration of HCC cells in vitro.According to the KEGG and GO enrichment analysis,in non-specific HCC,the genes co-expressed with SRP14 may predominantly regulate protein synthesis,processing,and transport,while in nonalcoholic fatty liver disease related HCC,the genes co-expressed with SRP14 could control multiple signaling pathways such as MAPK,cAMP,PI3K-Akt,and Wnt.Mechanistically,SRP14 up-regulated the mRNA expression of tumor suppressor gene GPRC5A in HCC cells(P<0.05).Conclusion:SRP14 may regulate HCC progression and influence patient prognosis.
