Berberine regulates glycemia via local inhibition of intestinal dipeptidyl peptidase-Ⅳ
10.3785/j.issn.1008-9292.2016.09.06
- VernacularTitle:小檗碱抑制肠道二肽基肽酶-Ⅳ的降糖机制研究
- Author:
Jiesheng WANG
1
;
Guanhai DAI
;
Weijia LI
Author Information
1. 浙江省立同德医院干部保健科
- Keywords:
Diabetes mellitus/drug therapy;
Berberine/pharmacology;
Berberine/administration & dosage;
Glucagon-like peptide 1/drug effects;
Antigens,CD26/drug effects;
Blood glucose/blood;
Diabetes mellitus,experimental
- From:
Journal of Zhejiang University. Medical sciences
2016;45(5):485-491
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of berberine on glycemia regulation in rats with diabetes and the related mechanisms .Methods: Diabetic-like rat model was successfully induced by intraperitoneal injection of streptozotocin in 50 out of 60 male SD rats, which were then randomly divided into 5 groups with 10 rats in each:control group (received vehicle only), positive drug control group (sitagliptin 10 mg· kg-1 · d-1 ) , low-dose berberine group ( 30 mg · kg-1 · d-1 ) , moderate-dose berberine group (60 mg· kg-1· d-1), and high-dose berberine group (120 mg· kg-1· d-1).All animals were fed for 3 d, and fasting blood sampling was performed on day 3 of administration .Rats were given glucose (2 g/kg) by gavage 30 min after the last dose . Blood and intestinal samples were obtained 2 h after glucose loading .Fasting blood glucose ( FBG) and 2-h postprandial plasma glucose (2h-PPG) were detected by using biochemical analyzer , and insulin , glucagon-like peptide-1 ( GLP-1 ) and dipeptidyl peptidase-Ⅳ( DPP-Ⅳ) were measured by using ELISA kit .Results: No significant difference in FBG and serum DPP-Ⅳ level were found between berberine groups and control group ( all P>0.05 ) .Compared with control group , serum levels of GLP-1 and insulin were increased in high-and moderate-dose berberine groups , while 2 h-PPG was decreased ( all P<0.05 );GLP-1 levels in the intestinal samples were increased , while DPP-Ⅳlevels were decreased in all berberine groups ( all P<0.05 ) .Conclusions:Short-term berberine administration can decrease 2h-PPG level in streptozotocin-induced diabetic rat model through local inhibition of intestinal DPP-Ⅳ.The efficacy of DPP-Ⅳinhibitor may be associated with its intestinal pharmacokinetics .
