Molecular epidemiological characterization of group A rotavirus in domestic sewage in Jinan from 2016 to 2018
10.3760/cma.j.cn112150-20200420-00606
- VernacularTitle:2016—2018年济南市生活污水轮状病毒A组分子流行病学特征分析
- Author:
Haibo DU
1
;
Xiaojuan LIN
;
Shanshan LIU
;
Feng JI
;
Zexin TAO
;
Yanyan SONG
;
Aiqiang XU
Author Information
1. 山东大学齐鲁医学院公共卫生学院，济南 250012
- Keywords:
Rotavirus;
Molecular epidemiology;
Environmental monitoring
- From:
Chinese Journal of Preventive Medicine
2020;54(10):1115-1120
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the detection of group A rotavirus (RVA) in domestic sewage and its molecular epidemiological characteristics, and further explore the feasibility and necessity of RVA environmental surveillance.Methods:From 2016 to 2018, we collected domestic sewage samples monthly in Jinan city, and concentrated them via anion membrane adsorption-elution method. Then RNA extraction and RVA VP7 and VP4 coding region RT-PCR amplification were performed. After purification, TA cloning and sequencing, homology analysis and phylogenetic analysis were conducted on the obtained sequences.Results:RVA G gene was detected in 31 of the 36 sewage samples (86.1% detection rate); RVA P genotype was detected in 33 samples (91.7% detection rate). A total of 536 RVA sequences were obtained, of which 225 G-type sequences belonged to 6 genotypes, and the G9 accounted for 92.4% (208/225); 311 P-type sequences were obtained, which belonged to 4 genotypes. The dominant P[8] accounted for 50.1% (156/311), followed by P[4] with 41.8% (130/311). Phylogenetic analysis shows that there were multiple transmission chains circulating in the dominant genotypes G9 and P[8].Conclusion:The genotype, homology, and phylogenetic characteristics of sequences obtained from domestic sewage in Jinan area were described, which further confirmeing that RVA environmental surveillance is not feasible but also necessary.
