Clinical applications of metagenomics next-generation sequencing in infectious diseases
- Author:
LIU YING
1
;
MA YONGJUN
Author Information
- Keywords: Metagenomics next-generation sequencing(mNGS); Infectious disease; Cerebrospinal fluid(CSF); Oxford Nanopore Technologies(ONT); Microbiome
- From: Journal of Zhejiang University. Science. B 2024;25(6):471-484
- CountryChina
- Language:Chinese
- Abstract: Infectious diseases are a great threat to human health.Rapid and accurate detection of pathogens is important in the diagnosis and treatment of infectious diseases.Metagenomics next-generation sequencing(mNGS)is an unbiased and comprehensive approach for detecting all RNA and DNA in a sample.With the development of sequencing and bioinformatics technologies,mNGS is moving from research to clinical application,which opens a new avenue for pathogen detection.Numerous studies have revealed good potential for the clinical application of mNGS in infectious diseases,especially in difficult-to-detect,rare,and novel pathogens.However,there are several hurdles in the clinical application of mNGS,such as:(1)lack of universal workflow validation and quality assurance;(2)insensitivity to high-host background and low-biomass samples;and(3)lack of standardized instructions for mass data analysis and report interpretation.Therefore,a complete understanding of this new technology will help promote the clinical application of mNGS to infectious diseases.This review briefly introduces the history of next-generation sequencing,mainstream sequencing platforms,and mNGS workflow,and discusses the clinical applications of mNGS to infectious diseases and its advantages and disadvantages.
