Influence of CO2 pneumoperitoneum on intracellular pH and signal transduction in cancer cells
- Author:
Li-Ping CAO
1
;
Guo-Ping DING
;
Ri-Sheng QUE
;
Shu ZHENG
Author Information
1. 浙江大学附属第二医院
- Keywords:
Cancer;
CO2 pneumoperitoneum;
Intracellular pH;
Signal transduction
- From:
Journal of Zhejiang University. Science. B
2005;6B(7):650-655
- CountryChina
- Language:Chinese
-
Abstract:
Object: The authors studied the influence of CO2 pneumoperitoneum on intracellular pH and signal transduction arising from cancer cell multiplication in laparoscopic tumor operation. Method: They set up a simulation of pneumoperitoneum under different CO2 pressure, and then measured the variation of intracellular pH (pHi) at different time and the activity of protein kinase C (PKC) and protein phosphatase 2a (PP2a) at the end of the pneumoperitoneum. After 1 week, the concentration of cancer cells in the culture medium was calculated. Result: When the pressure of CO2 pneumoperitoneum was 0, 10, 20, 30 mmHg respectively, the average pHi was 7.273, 7.075, 6.783, 6.693 at the end of the pneumoperitoneum; PKC activity was 159.4, 168.5,178.0, 181.6 nmol/(g.min) and PP2a was 4158.3, 4066.9, 3984.0, 3878.5 nmol/(g.min) respectively. After 1 week, the cancer cells concentration was 2.15×105, 2.03×105, 2.20×105, 2.18×105 L-1. Conclusion: CO2 pneumoperitoneum could promote acidosis in cancer cells, inducing the activation of protein kinase C and deactivation of protein phosphatase 2a, but it could not accelerate the mitosis rate of the cancer cells.
