Heterogeneous Nuclear Ribonucleoprotein A2B1 Exerts a Regulatory Role in Lipopolysaccharide-stimulated 38B9 B Cell Activation.
- Author:
Jisang PARK
1
;
Chung Hyeon CHOE
;
Ju KIM
;
Jing Shian YANG
;
Jin Hyun KIM
;
Hyonseok JANG
;
Yong Suk JANG
Author Information
- Publication Type:Original Article
- Keywords: B cell; Heterogeneous-nuclear ribonucleoproteins; MHC class II molecule; NF-κB; Signal transduction
- MeSH: B-Lymphocytes; Cytoplasm; Heterogeneous-Nuclear Ribonucleoproteins*; Major Histocompatibility Complex; Protein Kinases; Proto-Oncogene Proteins c-akt; Receptors, Antigen, T-Cell; RNA, Small Interfering; Signal Transduction
- From:Immune Network 2017;17(6):437-450
- CountryRepublic of Korea
- Language:English
- Abstract: Major histocompatibility complex (MHC) class II molecules, which are recognized for their primary function of presenting an antigen to the T cell receptor, are involved in various signaling pathways in B cell activation. We identified heterogeneous nuclear ribonucleoprotein (hnRNP) A2B1 as an MHC class II molecule-associated protein involved in MHC class II-mediated signal transduction in lipopolysaccharide (LPS)-stimulated 38B9 B cells. Although the function of hnRNP A2B1 in the nucleus is primarily known, the level of hnRNP A2B1 in the cytoplasm was increased in LPS-stimulated 38B9 cells, while it was not detected in the cytoplasm of non-treated 38B9 cells. The silencing of hnRNP A2B1 expression using siRNA disturbed B cell maturation by regulation of mitogen-activated protein kinase signaling, NF-κB activation, and protein kinase B activation. These results suggest that hnRNP A2B1 is associated with MHC class II molecules and is involved in B cell activation signaling pathways in LPS-stimulated 38B9 cells.
