Expression and correlation of microRNA-27b, microRNA-221, microRNA-222 and vascular endothelial growth factor in keloids
10.3760/cma.j.cn114453-20220808-00246
- VernacularTitle:microRNA-27b、microRNA-221、microRNA-222和血管内皮生长因子在瘢痕疙瘩中的表达及相关性研究
- Author:
Xue’e ZHU
1
;
Fang XIANG
;
Dong LUO
;
Xiaojing KANG
;
Yuan DING
Author Information
1. 新疆维吾尔自治区人民医院皮肤性病科 新疆皮肤病临床医学研究中心 新疆皮肤病研究重点实验室(XJYS1707), 乌鲁木齐 830001
- Keywords:
Keloid;
microRNAs;
Vascular endothelial growth factor;
Correlation analysis
- From:
Chinese Journal of Plastic Surgery
2023;39(8):844-850
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of microRNA-27b (miR-27b), miR-221 and miR-222 in keloids and their relationships with vascular endothelial growth factor (VEGF).Methods:From January 2021 to January 2022, 36 cases of keloid tissue (keloid group), 36 cases of adjacent normal tissue (normal control group) and 12 cases of flat scars (flat scar group) were collected from Dermatology and Venereology Department of Xinjiang Uygur Autonomous Region People's Hospital. VEGF protein expression and microvessel density in the three groups were determined by immunohistochemistry, and the expression levels of miR-27b, miR-221 and miR-222 were detected by real-time quantitative PCR. SPSS 27.0 statistical software was used for statistical analysis. The normally distributed measurement data were expressed as Mean±SD. ANOVA was used for multi-group comparison, and the LSD method was used for pound-for-pair multiple comparisons. The measurement data of non-normal distribution were expressed as M ( Q1, Q3), and Kruskal-Wallis H test was used for the comparison between groups. Spearman correlation analysis was used for the correlation analysis among data. All tests were bilateral tests, and P<0.05 was considered statistically significant. Results:The levels of VEGF protein and microvessel count in the keloid group [0.28±0.08, (47.78±14.06) bar /×400 view] were significantly higher than those in normal control group [0.09±0.05, (10.25±5.08) bar /×400 view] and flat scar group [0.19±0.06, (23.75±7.94) bar /×400 view], the difference was statistically significant ( P<0.01). The expression level of miR-27b in keloid group [0.50 (0.32, 0.64)] was significantly lower than that in normal control group [0.69 (0.37, 1.69)] and flat scar group [1.35 (1.25, 1.74)], the difference was statistically significant ( P<0.01). There were no significant differences in the expression levels of miR-221 and miR-222 among the three groups ( P>0.05). Correlation analysis showed that miR-27b, miR-221, miR-222 were positively correlated with VEGF protein expression in keloid group ( r=0.36, 0.41, 0.37, P<0.05). Conclusion:The expression of miR-27b is different in keloid, flat scar and normal tissue. miR-27b, miR-221 and miR-222 are positively correlated with VEGF expression in keloid, suggesting that the miRNA/VEGF axis may play a key role in the occurrence and progression of keloid.
