Relationship between TET2 Mutations and Expression of 5hmC in Breast Cancer Tissues
- VernacularTitle:乳腺癌组织中 TET2突变与5 hmC 表达的关系
- Author:
Panpan GU
1
;
Xin LUO
;
Hongwei ZHANG
;
Li TAN
;
Hao CHEN
;
Lingchun KONG
;
Jianbo DIAO
;
Yujiang SHI
;
Duan MA
Author Information
1. 复旦大学生物医学研究院
- Keywords:
Ten-eleven translocation 2;
Breast cancer;
5hmC;
Mutations
- From:
Chinese Journal of Clinical Medicine
2015;(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the relationship between ten‐eleven translocation 2 (TET2) Mutations and Expression of 5hmC in breast cancer .Methods :TET2 mutations in breast cancer tissues from 100 patients were detected by Sanger method . The expression of 5hmC in breast cancer tissues was detected by immunohistochemistry . The relationship between TET2 mutations and expression of 5hmC was analyzed .Results :A total of 13 kinds of TET2 mutations were identified in 100 patients .Among them ,3 new types of mutations were identified in 4 patients . They were Ala341Val , Leu1622Phe and Pro1578Tyr .Pro1578Tyr was identified in two patients .And immunohistochemical analysis showed that there was a sharp reduction of 5hmC in breast cancer tissues with TET2 mutations than in breast cancer tissues without TET2 mutations . Conclusions :T ET2 mutations can lead to decrease of 5hmC level in breast cancer .
