Diagnostic Value of Rv0222/ESAT-6 Fusion Protein in Tuberculosis
- VernacularTitle:Rv0222／ESAT-6融合蛋白对结核病的诊断价值
- Author:
Huizhang ZHANG
1
;
Qiwen FAN
;
Hua YANG
;
Hongmei HUANG
;
Qiang FANG
Author Information
1. 复旦大学附属金山医院检验科
- Keywords:
Fusion protein;
Tuberculosis;
Rv0222/ESAT-6 fusion protein;
Enzyme-linked immunosorbent assay
- From:
Chinese Journal of Clinical Medicine
2014;(6):636-639
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the serodiagnostic value of Rv0222/ESAT-6 fusion protein in tuberculosis.Methods:By using purified recombinant Rv0222/ESAT-6 fusion protein as antigen and enzyme-labeled antibody as secondary antibody,the serum of patients with tuberculosis and patients with human immunodeficiency virus(HIV)accompanied by tuberculosis,non-tubercu-losis patients and healthy people were detected with enzyme-linked immunosorbent assay(ELISA).The diagnostic value of the fusion protein in tuberculosis was evaluated.Results:By setting T lymphocyte spot test of infection with Mycobacterium tuber-culosis (T-SPOT)as the gold standard,the sensitivity and specificity of ELISA,of which Rv0222/ESAT-6 fusion protein was used as the antigen,in the diagnosis of tuberculosis were 86% and 100%,respectively.There was no statistically significant difference in the positive rate of tuberculosis between T-SPOT and ELISA.Conclusions:The ELISA with Rv0222/ESAT-6 fu-sion protein as antigen,has certain application value in the serodiagnosis of tuberculosis.
