Induced cleft palat by Retinoic acid through altering the cell proliferation and apoptosis at the key stages of palatal development
10.3760/cma.j.issn.1009-4598.2016.03.014
- VernacularTitle:维甲酸改变腭突发育关键时期细胞增殖和凋亡导致腭裂
- Author:
Xiao HU
1
;
Yeyang LI
;
Min LIANG
;
Weihua LIN
Author Information
1. 510220,暨南大学医学院附属广州市红十字会医院烧伤整形科
- Keywords:
Cleft Palate;
Tretinoin;
Cell Proliferation;
Apoptosis
- From:
Chinese Journal of Plastic Surgery
2016;32(3):220-224
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of retinoic acid (RA) on the proliferation and apoptosis of mesenchyme and epithelium at the key stages of palatal development,and to determine the embryonic alterations associated with cleft palate.Methods 100 pregnant C57 females (E12) were equally divided into two groups.The experimental group was administered RA once (70 mg/kg) while the control group was administered corn oil only,and the heads of the embryos at E13.5-17.5 were collected and processed to paraffin.Sections were stained with hematoxylin and eosin for the morphological assessment of anterior,posterior,left and right palate,and BrdU and TUNEL assays for the detection of proliferation and survival of palatal mesenchyme and epithelium.Results Simple treatment of RA at 70 mg/kg caused an incidence of 100％ cleft palate.During E13.5-15.5,cell proliferation was significantly promoted on the anterior palatal mesenchyme in the RA group,while no difference for the posterior palatal mesenchyme.Besides,the cell proliferation on anterior and posterior epithelium was comparable between the control and RA group.Less apoptotic cells were observed on epithelium during El3.5-14.5 in the RA group.Conclusions RA induces excessive cell proliferation of palatal mesenchyme,causing abnormal vertical palatal growth and failure of palatal shelves contact and fusion,which finally lead to cleft palate.RA also increases epithelium apoptosis.
