The Effect of Quercetin on the BK(Ca) in Umbilical Cord Vein-derived Mesenchymal Stem Cells.
10.5625/lar.2010.26.2.203
- Author:
Kyoung Sun PARK
1
;
Yangmi KIM
Author Information
1. Department of Physiology, College of Medicine, Seoul National University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Human umbilical cord vein-derived mesenchymal cells;
quercetin;
BK(Ca)
- MeSH:
Cell Cycle;
Homeostasis;
Humans;
Inhibitory Concentration 50;
Mesenchymal Stromal Cells;
Quercetin;
Umbilical Cord
- From:Laboratory Animal Research
2010;26(2):203-209
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Recently, we demonstrated that a large conductance Ca2+-activated K+ channel (BK(Ca)) in human umbilical cord vein-derived mesenchymal cells (hUC-MSCs). In this study, we studied effects of quercetin, the flavonoid used for the modulator of cell cycle and the treatment of anti-tumor, on BK(Ca) in hUC-MSCs using single channel and/or whole cell configuration. Single channel conductance was 277.8+/-36.2 pS in hUC-MSCs and the value is similar to that of typical BK(Ca) channel reported in other cells. The single channel current was activated by increasing of intracellular Ca2+. Quercetin (30~100 micrometer) increased BK(Ca) in whole cell patch configuration whereas EGCG was not affected on the channel. The activation effect of quercetin on BK(Ca) channel was also observed in inside-out configuration. The quercetin-induced BK(Ca) was increased a concentration-dependent manner with an IC50 value of 19.9 micrometer. Taken together, BK(Ca) in hUC-MSCs can be an important target for the action of quercetin and the channels are partly may contribute to maintaining homeostasis by regulation of K ion flux in hUC-MSCs.
