Establishment of a Novel Chinese Human Lung Adenocarcinoma Cell Line CPA-Yang1 which Produces Highly Bone Metastases in Immunodeficient Mice
10.3779/j.issn.1009-3419.2009.07.020
- VernacularTitle:中国人肺腺癌细胞系CPA-Yang1的建立及其生物学特性
- Author:
YANG SHUNFANG
1
;
SU JIANZHONG
;
CAO JIE
;
ZHANG PEILING
;
LU JIANYING
;
XIE WENHUI
Author Information
1. 上海交通大学附属胸科医院
- Keywords:
Lung neoplasms;
Tumor ceil;
Metastatic model;
Nude mouse
- From:
Chinese Journal of Lung Cancer
2009;12(7):753-759
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Lung cancer is a common malignancy and is the major determinant of overall cancer mortality worldwidely. Approximately 70％ of lung cancer patients will die from metastatic diseases. The aim of this study is to establish a Chinese lung adenocarcinoma cell line with high metastasis potency for exploring the mechanism of occurrence and development in lung cancer. Methods The cell came from the pericardial effusion of a fifty-year old male patient with lung adenocarcinoma and the cells in primary culture were obtained successfully. Immunodeficient mice tumorigenidty was assayed. The cell growth curve was mappinged Analysis of chromosome karyotype was tested. Tumor marker was detected by radioim-munoassay.The gene expression was measured by real-time quantitative PCR. Results The first passage cells were planted in im-munodeficiant mice subcutaneously and the tumorigenesis rate was 100％ as well as later passages. Under the microscope, the cell showed larger and semi-suspension, semi-adherence. Approximately 0.8x 106 cancerous ceils were injected into left cardiac ventri-cle or tail vein of immunodeficient mice resulted start to appear lower limb paralysis and spine swelling deformation in the mice after inoculation two-three weeks. The bone metastasis rate was 90％ in the tumor bearing mice by bone acintigraphy and pathol-ogy and only pulmonary metastasis 10％ at the same time. The chromosome karyotype analysis of the cells was sub-triploid. The tumor marker CEA was detected in higher secretion by radioimmunoassay in the cell culture suspension. Quantitative real-time PCK was used to examined and compared SPC-A-1 lung adenocarcinoma, VEGF-C, IL-6,IL,-8, genes were overexpressed. The novel cell line was named CPA-Yang1. Conclusion Tne novel strain CPA-Yang1 is an parental cell with characteristics of bone metastasis of Chinese lung adenocarcinoma. It has stable traits, highly metastatic ability and a good experimental modal for lungcancer research.
