Lipidomics Study of Huashi Baidu Granules in the Treatment of Respiratory Syncytial Virus Pneumonia Model Mice
10.14148/j.issn.1672-0482.2024.0359
- VernacularTitle:化湿败毒颗粒治疗呼吸道合胞病毒肺炎模型小鼠的脂质组学研究
- Author:
Bin YANG
1
;
Yu TANG
;
Chen SHI
;
Jinjun SHAN
Author Information
1. 南京中医药大学中医儿科学研究所,儿童健康与中医药江苏省高校重点实验室,江苏 南京 210023;南京中医药大学医学代谢组学中心,江苏 南京 210023
- Keywords:
respiratory syncytial virus;
pneumonia;
Huashi Baidu Granules;
lipidomics
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2024;40(4):359-368
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the therapeutic effect of Huashi Baidu Granules on respiratory syncytial virus(RSV)pneumonia model mice and its effect on lung lipid metabolism.METHODS BALB/c mice were randomly divided into control group,model group,Ribavirin group,Dexamethasone group,low dose group of Huashi Baidu Granules and high dose group of Huashi Baidu Granules.Lung tissue samples of mice in each group were collected to observe pathological changes and detect mRNA expression levels of RSV-F,RSV-G,IL-1β,IL-6 and TNF-α genes.The lipid components of lung tissue were extracted and analyzed by ultra-high performance liquid chromatography-quadrupole-electrostatic field orbital trap high-resolution mass spectrometry(UPLC-Q Exactive Orbitrap MS)to find the changes in lipid metabolism.RESULTS The model group exhibited pulmonary interstitial thickening and in-flammatory infiltration compared to the control group,the mRNA levels of viral load and inflammatory factors in lung tissue such as IL-1β and TNF-α were significantly increased(P<0.001).Moreover,the model group exhibited significant abnormalities in lipid metabo-lism,including disorders in phosphatidylserine(PS),phosphatidylglycerol(PG),sphingomyelin(SM),ceramide(Cer),triglyceride(TG),diglyceride(DG),fatty acid(FA),ether phosphatidylcholine(PC O),ether lysophosphatidylcholine(LPC O).However,af-ter the treatment of Huashi Baidu Granules(25.12 g·kg-1·d-1),the above indexes showed a significant improvement.CONCLU-SION Huashi Baidu Granules can treat RSV pneumonia by regulating lipid metabolism,reducing viral load and alleviating inflamma-tory response in lung tissue.
