Based on HMGB1/NF-κB Signal Pathway to Explore Inhibitory Effect of Wenjing Tongluo Decoction on IL-1β-Induced In-flammation in Primary Chondrocytes in Mice
10.14148/j.issn.1672-0482.2023.1095
- VernacularTitle:基于HMGB1/NF-κB通路探讨温经通络汤对IL-1β诱导小鼠原代软骨细胞炎症的影响
- Author:
Qi XU
1
;
Hong-Rong ZHANG
;
Wei-Min XU
;
Jia-Jia QIAN
;
Gui-Cheng HUANG
Author Information
1. 南京中医药大学第一临床医学院,江苏 南京 210023;安徽中医药大学中医学院,安徽 合肥 230038
- Keywords:
Wenjing Tongluo Decoction;
chondrocyte inflammation;
osteoarthritis;
HMGB1;
NF-κB signaling pathway
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2023;39(11):1095-1101
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To observe the effect of Wenjing Tongluo Decoction on IL-1β-induced inflammation of articular chon-drocytes and explore its mechanism.METHODS Primary chondrocytes were extracted from knee cartilage of mice and identified by toluidine blue staining.The chondrocyte inflammation model was induced by IL-1β.Lentiviral RNAi-HMGB1 was transfected into chondrocytes to interfere the expression of HMGB1.Then the cells were intervened with different concentrations of Wenjing Tongluo Decoction containing serum.Western Blot was used to analyze the protein expression of HMGB1 and NF-κB signaling pathway.The ex-pression of HMGB1,NF-κB1,NF-κB2 mRNA was detected by qPCR.ELISA was applied to detect the expression of inflammatory fac-tors IL-6,PGE-2 and TNF-α in cell supernatants.RESULTS Compared with normal cultured cells,the expression of HMGB1 in-creased significantly(P<0.01)in the IL-1β-induced chondrocyte inflammatory model,and the relative expression of p-IκBα/IκBα and p-P65/P65 proteins and the expression of NF-κB1 and NF-κB2 mRNA were increased(P<0.01).Compared with the model group,lentiviral RNAi-HMGB1 transfection could significantly downregulate the expression of HMGB1(P<0.01),and inhibit the upregulation of the relative expression of p-IκBα/IκBα(P<0.01)and p-P65/P65 protein and NF-κB1 and NF-κB2 mRNA expression(P<0.05).Compared with the model group,different concentrations of Wenjing Tongluo Decoction showed inhibitory effects on HMGB1 and NF-κB signaling pathways,demonstrated by the decrease in the protein expression of HMGB1,p-IκBα/IκBα and p-P65/P65(P<0.05).CONCLUSION Wenjing Tongluo Decoction can improve IL-1β-induced chondrocyte inflammation,and its mechanism may be related to inhibition of HMGB1-mediated activation of NF-κB signaling pathway.
