Puerarin Ameliorates Hyperglycemia in HFD Mice Dependent on Activation of GLP-1R Signaling Pathway
10.14148/j.issn.1672-0482.2017.0587
- VernacularTitle:葛根素激活GLP-1R通路改善高脂诱导的HFD小鼠高血糖水平
- Author:
Chun-Jun WANG
1
;
Lin-Jie JU
;
Xiao-Hua WEN
;
Jia-Li WANG
;
Luan SHU
Author Information
1. 安徽中医药大学药学院
- Keywords:
Exendin 9-39;
GLP-1R;
puerarin;
type 2 diabetes
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2017;33(6):587-590
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effect of puerarin on hyperglycemia in HFD mice and the role of GLP-1R signa-ling in modulating the hypoglycemic effect of puerarin.METHODS HFD induced diabetic mice was used as the subject,GLP-1 receptor antagonist Exendin 9-39(Ex 9-39)was used to block the activation of GLP-1R signaling,and in vivo experiments were performed to further evaluate the role of GLP-1R signaling in the hypoglycemic effect of puerarin.RESULTS The glu-cose homeostasis and the OGTT(oral glucose tolerance tests)of HFD mice were both improved by puerarin,while the effect was impaired by Ex 9-39 significantly.Moreover,puerarin increased serum insulin contents in HFD mice,but such effect was blocked by Ex 9-39 as well.Elevated expression of ki67 inβ-cells was observed in pancreatic sections from HFD mice pre-trea-ted with puerarin using Ki-67/insulin immunostaining,suggesting that puerarin could promoteβ-cell proliferation.The puerar-in effect was inhibited when the GLP-1R signaling activation was blocked by Ex9-39.CONCLUSION The results confirmed that the activation of GLP-1R signaling was critical for the anti-diabetic effects of puerarin on HFD induced diabetic mice.Our finding highlights the potential value of Radix puerariae as a dietary supplement for diabetes care.
