Etiological diagnostic methods and research progress of forest encephalitis
10.3760/cma.j.cn121094-20230703-00227
- VernacularTitle:森林脑炎的病原学诊断方法及研究进展
- Author:
Dongmei SHI
1
;
Li SONG
;
Ji WANG
Author Information
1. 黑龙江省劳动卫生职业病研究院职业中毒科，哈尔滨 150028
- Keywords:
Encephalitis, tick-borne;
Forest encephalitis;
Serologic testing;
Molecular biology testing;
Metabolomics;
Digital PCR;
Matrix metalloproteinases
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2024;42(2):152-155
- CountryChina
- Language:Chinese
-
Abstract:
Forest encephalitis is a natural focal disease transmitted through the bite of hard ticks, and its pathogen is the tick-borne encephalitis virus from the Flaviviridae family. The mortality rate of forest encephalitis is relatively high, making laboratory testing significant in diagnosing this disease. This article elaborates on the etiological diagnostic methods and recent research progress in forest encephalitis. Laboratory tests for forest encephalitis mainly include routine examinations, serological tests, virus isolation, and molecular biological testing. The detection of serum-specific IgM antibodies against the forest encephalitis virus is of great importance for early diagnosis, and specific IgG antibodies serve as a "gold standard" for differentiation from other diseases. Techniques such as enzyme-linked immunosorbent assay (ELISA) or indirect immunofluorescence assay for detecting specific IgM antibodies in serum and/or cerebrospinal fluid, the serum hemagglutination inhibition test or serum complement fixation test, and the double serum hemagglutination inhibition test or complement fixation test all contribute to the early diagnosis. The development of molecular testing methods is rapid, and techniques such as metabolomics, digital PCR, and matrix metalloproteinases are also applied in the early diagnosis of forest encephalitis.
